Coding

Part:BBa_K3385040

Designed by: Lucas Levassor   Group: iGEM20_DTU-Denmark   (2020-10-12)


Gene_glaA

Theoretical expectation: This part encodes the native Glucoamylase (glaA) found in A. niger.


Functionality: Glucoamylase is an enzyme that hydrolyzes the terminal 1 -> 4 linked alpha-D-glucose from the non-reducing end of starch.


Results: Below is a picture showing A. niger grown on starch plates with subsequent addition of iodine yielding no colouring zones showing breakdown of starch.

Starch/Iodine plate assay of the ATCC 1015 strain and a K/O (glaA) strain. A clear halo is seen were starch has been consumed since iodine binds starch.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 879
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1756
    Illegal PstI site found at 879
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 734
    Illegal BamHI site found at 1714
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 879
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 879
    Illegal NgoMIV site found at 1974
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 262
    Illegal BsaI site found at 400
    Illegal BsaI site found at 1089
    Illegal BsaI.rc site found at 1582


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Categories
Parameters
None